Small Fluorogenic Amino Acids for Peptide-Guided Background-Free Imaging.

ABSTRACT: The multiple applications of super-resolution microscopy have prompted the need for minimally invasive labeling strategies for peptide-guided fluorescence imaging. Many fluorescent reporters display limitations (e.g., large and charged scaffolds, non-specific binding) as building blocks for the construction of fluorogenic peptides. Herein we have built a library of benzodiazole amino acids and systematically examined them as reporters for background-free fluorescence microscopy. We have identified amine-derivatized benzoselenadiazoles as scalable and photostable amino acids for the straightforward solid-phase synthesis of fluorescent peptides. Benzodiazole amino acids retain the binding capabilities of bioactive peptides and display excellent signal-to-background ratios. Furthermore, we ha

SUBMITTER: de Moliner F 

PROVIDER: S-EPMC10108274 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature