Secretory expression of recombinant small laccase genes in Gram-positive bacteria.

ABSTRACT:

Background

Laccases are multicopper enzymes that oxidize a wide range of aromatic and non-aromatic compounds in the presence of oxygen. The majority of industrially relevant laccases are derived from fungi and are produced in eukaryotic expression systems such as Pichia pastoris and Saccharomyces cerevisiae. Bacterial laccases for research purposes are mostly produced intracellularly in Escherichia coli, but secretory expression systems are needed for future applications. Bacterial laccases from Streptomyces spp. are of interest for potential industrial applications because of their lignin degrading activities.

Results

In this study, we expressed small laccases genes from Streptomyces coelicolor, Streptomyces viridosporus and Amycolatopsis 75iv2 with their native signal sequ

SUBMITTER: Valimets S 

PROVIDER: S-EPMC10108450 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature