Shengjiang San alleviated sepsis-induced lung injury through its bidirectional regulatory effect.

ABSTRACT:

Background

Sepsis is a life-threatening organ dysfunction caused by dysregulated host responses to infection, for which effective therapeutic strategies are still absent. Shengjiang San (SJS), a well-known Traditional Chinese Medicine formula, has been widely used clinically. However, its role in sepsis-induced lung injury remains unclear.

Methods

To explore its specific mechanism, we firstly established a sepsis animal model using cecal ligation and puncture (CLP) and treated MH-S cells with LPS plus ATP. Then, UPLC/Q-TOF-MS/MS was utilized to identify its active ingredients. Network pharmacology analysis was performed to uncover the potential mechanism. HE staining and biochemical analysis were conducted to validate its therapeutic effect. ELISA was applied to detect the r

SUBMITTER: Yan S 

PROVIDER: S-EPMC10108513 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature