Using RNA-seq to identify suitable housekeeping genes for hypoxia studies in human adipose-derived stem cells.

ABSTRACT:

Background

Hypoxic culture conditions have been used to study the impact of oxygen deprivation has on gene expression in a number of disease models. However, hypoxia response elements present in the promoter regions of some commonly used housekeeping genes, such as GAPDH and PGK1, can confound the relative gene expression analysis. Thus, there is ongoing debate as to which housekeeping gene is appropriate for studies investigating hypoxia-induced cell responses. Specifically, there is still contradicting information for which housekeeping genes are stable in hypoxia cultures of mesenchymal stem cells. In this study, candidate housekeeping genes curated from the literature were matched to RNAseq data of normoxic and hypoxic human adipose-derived stem cell cultures to determine if ge

SUBMITTER: Ong HT 

PROVIDER: S-EPMC10108514 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature