Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay.

ABSTRACT: Events associated with oocyte nuclear maturation have been well described. However, much less is known about the molecular pathways and processes that take place in the cytoplasm in preparation for fertilization and acquisition of totipotency. During oocyte maturation, changes in gene expression depend exclusively on the translation and degradation of maternal messenger RNAs (mRNAs) rather than on transcription. Execution of the translational program, therefore, plays a key role in establishing oocyte developmental competence to sustain embryo development. This paper is part of a focus on defining the program of maternal mRNA translation that takes place during meiotic maturation and at the oocyte-to-zygote transition. In this method paper, a strategy is presented to study the regulation o

SUBMITTER: Costermans NGJ 

PROVIDER: S-EPMC10108655 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature