Tissue clearing and immunostaining to visualize the spatial organization of vasculature and tumor cells in mouse liver.

ABSTRACT: The liver has a complex and hierarchical segmental organization of arteries, portal veins, hepatic veins and lymphatic vessels. In-depth imaging of liver vasculature and malignancies could improve knowledge on tumor micro-environment, local tumor growth, invasion, as well as metastasis. Non-invasive imaging techniques such as computed tomography (CT), magnetic resonance imaging (MRI) and positron-emission transmission (PET) are routine for clinical imaging, but show inadequate resolution at cellular and subcellular level. In recent years, tissue clearing - a technique rendering tissues optically transparent allowing enhanced microscopy imaging - has made great advances. While mainly used in the neurobiology field, recently more studies have used clearing techniques for imaging other organ

SUBMITTER: Frenkel N 

PROVIDER: S-EPMC10108913 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature