Inferring assembly-curving trends of bacterial micro-compartment shell hexamers from crystal structure arrangements.

ABSTRACT: Bacterial microcompartments (BMC) are complex macromolecular assemblies that participate in varied chemical processes in about one fourth of bacterial species. BMC-encapsulated enzymatic activities are segregated from other cell contents by means of semipermeable shells, justifying why BMC are viewed as prototype nano-reactors for biotechnological applications. Herein, we undertook a comparative study of bending propensities of BMC hexamers (BMC-H), the most abundant shell constituents. Published data show that some BMC-H, like β-carboxysomal CcmK, tend to assemble flat whereas other BMC-H often build curved objects. Inspection of available crystal structures presenting BMC-H in tiled arrangements permitted us to identify two major assembly modes with a striking connection with experimenta

SUBMITTER: Garcia-Alles LF 

PROVIDER: S-EPMC10109471 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature