Phosphatidylinositol metabolism of the renal proximal tubule S3 segment is disturbed in response to diabetes.

ABSTRACT: Diabetes is a main risk factor for kidney disease, causing diabetic nephropathy in close to half of all patients with diabetes. Metabolism has recently been identified to be decisive in cell fate decisions and repair. Here we used mass spectrometry imaging (MSI) to identify tissue specific metabolic dysregulation, in order to better understand early diabetes-induced metabolic changes of renal cell types. In our experimental diabetes mouse model, early glomerular glycocalyx barrier loss and systemic metabolic changes were observed. In addition, MSI targeted at small molecule metabolites and glycero(phospho)lipids exposed distinct changes upon diabetes in downstream nephron segments. Interestingly, the outer stripe of the outer medullar proximal tubular segment (PT_S3) demonstrated the most

SUBMITTER: Rietjens RGJ 

PROVIDER: S-EPMC10110589 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature