Efficiency scale for scattering luminescent particles linked to fundamental and measurable spectroscopic properties.

ABSTRACT: Comparing the performance of molecular and nanoscale luminophores and luminescent micro- and nanoparticles and estimating achievable signal amplitudes and limits of detection requires a standardizable intensity scale. This initiated the development of the relative MESF (number of molecules of equivalent soluble fluorochromes) and ERF (equivalent reference fluorophores) scales for flow cytometry and fluorescence microscopy. Both intensity scales rely on fluorescence intensity values assigned to fluorescent calibration beads by an intensity comparison to spectrally closely matching fluorophore solutions of known concentration using a spectrofluorometer. Alternatively, the luminophore or bead brightness (B) can be determined that equals the product of the absorption cross section (σ_a

SUBMITTER: Wurth C 

PROVIDER: S-EPMC10110600 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature