Remodeling of yeast vacuole membrane lipidomes from the log (one phase) to stationary stage (two phases).

ABSTRACT: Upon nutrient limitation, budding yeast of Saccharomyces cerevisiae shift from fast growth (the log stage) to quiescence (the stationary stage). This shift is accompanied by liquid-liquid phase separation in the membrane of the vacuole, an endosomal organelle. Recent work indicates that the resulting micrometer-scale domains in vacuole membranes enable yeast to survive periods of stress. An outstanding question is which molecular changes might cause this membrane phase separation. Here, we conduct lipidomics of vacuole membranes in both the log and stationary stages. Isolation of pure vacuole membranes is challenging in the stationary stage, when lipid droplets are in close contact with vacuoles. Immuno-isolation has previously been shown to successfully purify log-stage vacuole membranes

SUBMITTER: Reinhard J 

PROVIDER: S-EPMC10111276 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature