Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility.

ABSTRACT: Essential cellular processes such as metabolism, protein synthesis, and autophagy require the intracellular transport of membrane-bound vesicles. The importance of the cytoskeleton and associated molecular motors for transport is well documented. Recent research has suggested that the endoplasmic reticulum (ER) may also play a role in vesicle transport through a tethering of vesicles to the ER. We use single-particle tracking fluorescence microscopy and a Bayesian change-point algorithm to characterize vesicle motility in response to the disruption of the ER, actin, and microtubules. This high-throughput change-point algorithm allows us to efficiently analyze thousands of trajectory segments. We find that palmitate-mediated disruption of the ER leads to a significant decrease in vesicle mo

SUBMITTER: Rayens NT 

PROVIDER: S-EPMC10111363 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature