NLRP3 Inflammasome Activation in Peripheral Arterial Disease.

ABSTRACT: Background Peripheral arterial disease (PAD) is estimated to affect 7% of the adult population in the United States; however, there is currently little understanding of the key cellular and molecular pathways at play. With PAD characterized by vascular inflammation and associated calcification, the current study set out to elucidate the role of NLRP3 (nucleotide oligomerization domain-like receptor family, pyrin domain containing 3) inflammasome activation in the current cohort. Methods and Results Global proteomics of human vessels with and without PAD from a total of 14 donors revealed an increase of proinflammatory associated ontologies, specifically acute phase and innate immunity. Targeted mass spectrometry showed a significant increase in NLRP3, confirmed by NLRP3 ELISA. Histological

SUBMITTER: Bartoli-Leonard F 

PROVIDER: S-EPMC10111545 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature