Regulation of Miwi-mediated mRNA stabilization by Ck137956/Tssa is essential for male fertility.

ABSTRACT:

Background

Sperm is formed through spermiogenesis, a highly complex process involving chromatin condensation that results in cessation of transcription. mRNAs required for spermiogenesis are transcribed at earlier stages and translated in a delayed fashion during spermatid formation. However, it remains unknown that how these repressed mRNAs are stabilized.

Results

Here we report a Miwi-interacting testis-specific and spermiogenic arrest protein, Ck137956, which we rename Tssa. Deletion of Tssa led to male sterility and absence of sperm formation. The spermiogenesis arrested at the round spermatid stage and numerous spermiogenic mRNAs were down-regulated in Tssa^-/- mice. Deletion of Tssa disrupted the localization of Miwi to chromatoid body, a specialized assembly

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10111675 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature