A Novel Anti-CRISPR AcrIE9.2 Is Associated with Dissemination of bla_KPC Plasmids in Klebsiella pneumoniae Sequence Type 15.

ABSTRACT: Sequence type (ST) 15 has become an emerging clone of carbapenem-resistant Klebsiella pneumoniae in which type I-E* CRISPR-Cas usually exists, indicating that the CRISPR-Cas system may not be able to block the transfer of bla_KPC plasmids. The purpose of this study was to explore the mechanisms underlying dissemination of bla_KPC plasmids in K. pneumoniae ST15. The type I-E* CRISPR-Cas system was present in 98.0% of 612 nonduplicate K. pneumoniae ST15 strains (88 clinical isolates and 524 from the NCBI database). Twelve ST15 clinical isolates were completely sequenced, and self-targeted protospacers were found on bla_KPC plasmids flanked by a protospacer adjacent motif (PAM) of AAT in 11 isolates. The type I-E* CRISPR-Cas system was cloned

SUBMITTER: Wang C 

PROVIDER: S-EPMC10112057 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature