Dataset Information

A study on genetic and mutans streptococcal transmissibility of dental caries.

ABSTRACT:

Background

Dental caries is characterized by an interplay between environmental and genetic factors.

Aim

The aim of this study was to analyse the transmissibilities of high caries risk chromosomal loci at 5q 12.1-13.3 and low caries risk chromosomal loci at 13q31.1 and Streptococcus mutans (S. mutans) in family units.

Materials

This prospective cohort study was performed on 56 families grouped into four: (a) Group I: 18 families of children with caries affected primary teeth; (b) Group II: 21 families of children with caries in permanent teeth; (c) Group III: 6 families of children with no caries in primary teeth and (d) Group IV: 12 families of children with no caries in permanent teeth. Blood, saliva and plaque samples were collected from consenting study participants. Isolated DNAs were subjected to polymerase chain reactions using suitable primers. Data collected was analysed with ANOVA and Chi-squared test.

Results

Wide expression of chromosome loci 5q12.1-13.3 was obtained in both blood and saliva samples. For chromosome loci 13q31.1, no expression was found in saliva samples, hence indicating its local absence. For the GtfB expression, transmissibility was common for a single band expressing S. mutans.

Conclusion

This study reflects upon newer findings in the field of genetic research on dental caries.

SUBMITTER: Chatterjee S

PROVIDER: S-EPMC10112085 | biostudies-literature | 2022 Oct-Dec

REPOSITORIES: biostudies-literature

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Publications

A study on genetic and mutans streptococcal transmissibility of dental caries.

Chatterjee Shailja S Damle Satyawan G SG Iyer Nageshwar N

Journal of oral and maxillofacial pathology : JOMFP 20221001 4

<h4>Background</h4>Dental caries is characterized by an interplay between environmental and genetic factors.<h4>Aim</h4>The aim of this study was to analyse the transmissibilities of high caries risk chromosomal loci at 5q 12.1-13.3 and low caries risk chromosomal loci at 13q31.1 and Streptococcus mutans (S. mutans) in family units.<h4>Materials</h4>This prospective cohort study was performed on 56 families grouped into four: (a) Group I: 18 families of children with caries affected primary teet ...[more]

PMID: 37082046

Similar Datasets

Project description:ObjectivesLactobacilli and Streptococcus mutans are stigmatized as cariogenic bacteria, but few studies have simultaneously examined the quantitative and qualitative aspects of lactobacilli and S. mutans in childhood dental caries. Therefore, this study aimed to detect the presence of S. mutans and lactobacilli in dental caries using Snyder's test, colony counting, and PCR in the primary teeth of Iranian children with dental caries.Material and methodsA cross-sectional study was conducted in Kerman, Iran, from March to Jun 2024. After dental examinations, 120 eligible children aged 5-9 were chosen using simple random sampling and classified into four groups based on their decayed, missing, and filled (primary) teeth (dmft) index: Group 1 (dmft = 0), Group 2 (dmft = 4-6), Group 3 (dmft = 7-9), and Group 4 (dmft = 10-13). The salivary levels of lactobacilli and S. mutans were calculated using colony counting (CFU/ml). Moreover, Snyder's test was applied to evaluate caries activity. PCR was also performed for molecular detection of lactobacilli (16S rRNA gene) and S. mutans (gftB gene). Lastly, the association between bacterial counting, molecular findings, and Snyder's test was estimated through statistical methods using SPSS 27.ResultsSignificant differences were found between the age and the PCR results of lactobacilli and S. mutans among all dmft Groups (p < 0.05). Moreover, positive significant correlations were observed between the counts of lactobacilli and S. mutans in dmft Group 1 compared to other dmft Groups (p < 0.05). Furthermore, the counts of these two bacteria differed significantly in Snyder's test (p < 0.05). However, Snyder's test differed significantly from the S. mutans PCR (p < 0.05), but not from the lactobacilli PCR (p > 0.05).ConclusionsThe results of the study could potentially be considered a promising and cost-effective screening program to identify children who are susceptible to dental caries.

Dataset Information

A study on genetic and mutans streptococcal transmissibility of dental caries.

Background

Aim

Materials

Results

Conclusion

Publications

A study on genetic and mutans streptococcal transmissibility of dental caries.

Similar Datasets

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