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Complete Genomic Analysis of the Novel Phage BUCT-3589, Infecting Klebsiella pneumoniae.

ABSTRACT: We report the complete genome sequence of the phage BUCT-3589, infecting multidrug-resistant Klebsiella pneumoniae 3589. It is a new member of the genus Przondovirus in the family Autographiviridae and possesses a double-stranded DNA (dsDNA) genome of 40,757 bp with 53.13% GC content. The genome sequence will support its use as a therapeutic agent.

SUBMITTER: Zhang X

PROVIDER: S-EPMC10112201 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Complete Genomic Analysis of the Novel Phage BUCT-3589, Infecting Klebsiella pneumoniae.

Zhang Xiaoxuan X Mi Yanze Y Xu Shan S Song Lihua L Fan Huahao H Li Mengzhe M Tong Yigang Y

Microbiology resource announcements 20230302 4

We report the complete genome sequence of the phage BUCT-3589, infecting multidrug-resistant Klebsiella pneumoniae 3589. It is a new member of the genus <i>Przondovirus</i> in the family <i>Autographiviridae</i> and possesses a double-stranded DNA (dsDNA) genome of 40,757 bp with 53.13% GC content. The genome sequence will support its use as a therapeutic agent. ...[more]

PMID: 36861969

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Project description:BackgroundBacteriophages (phages) are one of the most promising alternatives to traditional antibiotic therapies, especially against multidrug-resistant bacteria. Klebsiella pneumoniae is considered to be an opportunistic pathogen that can cause life-threatening infections. Thus, this study aims at the characterization of a novel isolated phage vB_Kpn_ZC2 (ZCKP2, for short).MethodsThe phage ZCKP2 was isolated from sewage water by using the clinical isolate KP/08 as a host strain. The isolated bacteriophage was purified and amplified, followed by testing of its molecular weight using Pulse-Field Gel Electrophoresis (PFGE), transmission electron microscopy, antibacterial activity against a panel of other Klebsiella pneumoniae hosts, stability studies, and whole genome sequencing.ResultsPhage ZCKP2 belongs morphologically to siphoviruses as indicated from the Transmission Electron Microscopy microgram. The Pulsed Field Gel Electrophoresis and the phage sequencing estimated the phage genome size of 48.2 kbp. Moreover, the absence of lysogeny-related genes, antibiotic resistance genes, and virulence genes in the annotated genome suggests that phage ZCKP2 is safe for therapeutic use. Genome-based taxonomic analysis indicates that phage ZCKP2 represents a new family that has not been formally rated yet. In addition, phage ZCKP2 preserved high stability at different temperatures and pH values (-20 - 70 °C and pH 4 - 9). For the antibacterial activity, phage ZCKP2 maintained consistent clear zones on KP/08 bacteria along with other hosts, in addition to effective bacterial killing over time at different MOIs (0.1, 1, and 10). Also, the genome annotation predicted antibacterial lytic enzymes. Furthermore, the topology of class II holins was predicted in some putative proteins with dual transmembrane domains that contribute significantly to antibacterial activity. Phage ZCKP2 characterization demonstrates safety and efficiency against multidrug-resistant K. pneumoniae, hence ZCKP2 is a good candidate for further in vivo and phage therapy clinical applications.

Dataset Information

Complete Genomic Analysis of the Novel Phage BUCT-3589, Infecting Klebsiella pneumoniae.

Publications

Complete Genomic Analysis of the Novel Phage BUCT-3589, Infecting Klebsiella pneumoniae.

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