8-Aminoguanine and Its Actions on Renal Excretory Function.

ABSTRACT:

Background

The endogenous purine 8-aminoguanine induces diuresis/natriuresis/glucosuria by inhibiting PNPase (purine nucleoside phosphorylase); however, mechanistic details are unknown.

Methods

Here, we further explored in rats 8-aminoguanine's effects on renal excretory function by combining studies using intravenous 8-aminoguanine, intrarenal artery infusions of PNPase substrates (inosine and guanosine), renal microdialysis, mass spectrometry, selective adenosine receptor ligands, adenosine receptor knockout rats, laser doppler blood flow analysis, cultured renal microvascular smooth muscle cells, HEK293 cells expressing A_2B receptors and homogeneous time resolved fluorescence assay for adenylyl cyclase activity.

Results

Intravenous 8-aminoguanine caused

SUBMITTER: Jackson EK 

PROVIDER: S-EPMC10112938 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature