The kinesin-5 protein Cut7 moves bidirectionally on fission yeast spindles with activity that increases in anaphase.

ABSTRACT: Kinesin-5 motors are essential to separate mitotic spindle poles and assemble a bipolar spindle in many organisms. These motors crosslink and slide apart antiparallel microtubules via microtubule plus-end-directed motility. However, kinesin-5 localization is enhanced away from antiparallel overlaps. Increasing evidence suggests this localization occurs due to bidirectional motility or trafficking. The purified fission-yeast kinesin-5 protein Cut7 moves bidirectionally, but bidirectionality has not been shown in cells, and the function of the minus-end-directed movement is unknown. Here, we characterized the motility of Cut7 on bipolar and monopolar spindles and observed movement toward both plus- and minus-ends of microtubules. Notably, the activity of the motor increased at anaphase B ons

SUBMITTER: Gergely ZR 

PROVIDER: S-EPMC10112985 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature