CircRBM33 promotes migration, invasion and mediates osimertinib resistance in non-small cell lung cancer cell line.

ABSTRACT:

Background

It has been shown that circular RNAs (circRNAs) are involved in the pathogenesis of non-small cell lung cancer (NSCLC). However, the molecular mechanisms of circRNAs in tumor malignant progression and tyrosine kinase inhibitors (TKI) resistance remain undefined. Hereby, we explored the mechanisms by which circRBM33 promotes NSCLC progression and epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKI) resistance.

Methods

Transcriptome sequencing (RNA-seq) was carried out to obtain the circRBM33 we investigated. Quantitative reverse transcriptase-polymerase chain reaction was performed to detect the expression of circRBM33. Cell counting kit-8 assay was performed to detect cell proliferation as well as flow cytometry to detect cel

SUBMITTER: Pan J 

PROVIDER: S-EPMC10113067 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature