LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p.

ABSTRACT:

Background

Long-non-coding RNA PVT1 (lncRNA PVT1) can be used as an oncogenic regulatory non-coding RNA (ncRNA) for many cancers. However, its function and mechanism in breast cancer (BRCA) are still not clearly elucidated.

Objective

We attempt to explain the mechanism of PVT1's role in breast cancer from different perspectives.

Methods

We analyzed the expression of PVT1 and its correlation with the breast cancer related clinical data in the The Cancer Genome Atlas (TCGA) database. We used PVT1 overexpression and knockdown lentivirus to infect breast cancer MDA-MB-231 cell line for cell function verification, in vitro using CCK-8 to measure proliferation, flow cytometry to measure apoptosis, transwell test to measure invasion and migration ability, detecting cell extr

SUBMITTER: Qu H 

PROVIDER: S-EPMC10113361 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature