Root endophytic microbiome dataset of sugarcane (Saccharum officinarum L.) cultivated in the Central Highlands, Vietnam, established by the 16S rRNA metagenomics.

ABSTRACT: Sugarcane (Saccharum officinarum L.) is one of the main annual crops grown in the Central Highlands of Vietnam. By understanding the taxonomic and functional profiles of root endophytic microbiome, we can develop a new cultivation technique for the sustainable production of this plant. In this work, a representative sample was obtained by mixing the roots collected from five different sugarcane fields in Dak Lak Province, the Central Highlands, in 2021. The genomic DNA of the endophytic bacteria was extracted using the DNeasy Powersoil kit, and the V1-V9 regions of the 16S rRNA genes were amplified by PCR. Libraries of 16S rRNA gene amplicons were prepared using the Swift amplicon 16S plus ITS panel kit. Finally, the Illumina MiSeq platform was used to sequence the 16S rRNA gene amp

SUBMITTER: Nguyen TH 

PROVIDER: S-EPMC10113743 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature