N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates.

ABSTRACT:

Background

Argonaute proteins play a central role in RNA silencing by forming protein-small RNA complexes responsible for the silencing process. While most Argonaute proteins have a short N-terminal region, Argonaute2 in Drosophila melanogaster (DmAgo2) harbors a long and unique N-terminal region. Previous in vitro biochemical studies have shown that the loss of this region does not impair the RNA silencing activity of the complex. However, an N-terminal mutant of Drosophila melanogaster has demonstrated abnormal RNA silencing activity. To explore the causes of this discrepancy between in vitro and in vivo studies, we investigated the biophysical properties of the region. The N-terminal region is highly rich in glutamine and glycine residues, which is a well-known property for prio

SUBMITTER: Narita H 

PROVIDER: S-EPMC10114355 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature