A guide to naming eukaryotic circular RNAs.
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ABSTRACT:
SUBMITTER: Chen LL
PROVIDER: S-EPMC10114414 | biostudies-literature | 2023 Jan
REPOSITORIES: biostudies-literature
Publications
A guide to naming eukaryotic circular RNAs.
Chen Ling-Ling LL Bindereif Albrecht A Bozzoni Irene I Chang Howard Y HY Matera A Gregory AG Gorospe Myriam M Hansen Thomas B TB Kjems Jørgen J Ma Xu-Kai XK Pek Jun Wei JW Rajewsky Nikolaus N Salzman Julia J Wilusz Jeremy E JE Yang Li L Zhao Fangqing F
Nature cell biology 20230101 1
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Project description:BackgroundThe CRISPR/Cas12a and CRISPR/Cas13d systems are widely used for fundamental research and hold great potential for future clinical applications. However, the short half-life of guide RNAs (gRNAs), particularly free gRNAs without Cas nuclease binding, limits their editing efficiency and durability.ResultsHere, we engineer circular free gRNAs (cgRNAs) to increase their stability, and thus availability for Cas12a and Cas13d processing and loading, to boost editing. cgRNAs increases the efficiency of Cas12a-based transcription activators and genomic DNA cleavage by approximately 2.1- to 40.2-fold for single gene editing and 1.7- to 2.1-fold for multiplexed gene editing than their linear counterparts, without compromising specificity, across multiple sites and cell lines. Similarly, the RNA interference efficiency of Cas13d is increased by around 1.8-fold. In in vivo mouse liver, cgRNAs are more potent in activating gene expression and cleaving genomic DNA.ConclusionsCgRNAs enable more efficient programmable DNA and RNA editing for Cas12a and Cas13d with broad applicability for fundamental research and gene therapy.
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