Phenotyping the Immune Response to Trauma: A Multiparametric Systems Immunology Approach.

ABSTRACT:

Objective

Trauma induces a complex immune response that requires a systems biology research approach. Here, we used a novel technology, mass cytometry by time-of-flight, to comprehensively characterize the multicellular response to trauma.

Design

Peripheral blood mononuclear cells samples were stained with a 38-marker immunophenotyping cytometry by time-of-flight panel. Separately, matched peripheral blood mononuclear cells were stimulated in vitro with heat-killed Streptococcus pneumoniae or CD3/CD28 antibodies and stained with a 38-marker cytokine panel. Monocytes were studied for phagocytosis and oxidative burst.

Setting

Single-institution level 1 trauma center.

Patients or subjects

Trauma patients with injury severity scores greater than 20 (n = 10) at days

SUBMITTER: Seshadri A 

PROVIDER: S-EPMC10114604 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature