Cell-free circulating tumor RNAs in plasma as the potential prognostic biomarkers in colorectal cancer.

ABSTRACT:

Background

Cell free RNA (cfRNA) contains transcript fragments from multiple cell types, making it useful for cancer detection in clinical settings. However, the pathophysiological origins of cfRNAs in plasma from colorectal cancer (CRC) patients remain unclear.

Methods

To identify the tissue-specific contributions of cfRNAs transcriptomic profile, we used a published single-cell transcriptomics profile to deconvolute cell type abundance among paired plasma samples from CRC patients who underwent tumor-ablative surgery. We further validated the differentially expressed cfRNAs in 5 pairs of CRC tumor samples and adjacent tissue samples as well as 3 additional CRC tumor samples using RNA-sequencing.

Results

The transcriptomic component from intestinal secretory cells wa

SUBMITTER: Jin N 

PROVIDER: S-EPMC10115432 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature