Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens.

ABSTRACT: Expansion microscopy physically enlarges biological specimens to achieve nanoscale resolution using diffraction-limited microscopy systems¹. However, optimal performance is usually reached using laser-based systems (for example, confocal microscopy), restricting its broad applicability in clinical pathology, as most centres have access only to light-emitting diode (LED)-based widefield systems. As a possible alternative, a computational method for image resolution enhancement, namely, super-resolution radial fluctuations (SRRF)^2,3, has recently been developed. However, this method has not been explored in pathology specimens to date, because on its own, it does not achieve sufficient resolution for routine clinical use. Here, we report expansion-enhanced super-resolut

SUBMITTER: Kylies D 

PROVIDER: S-EPMC10115634 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature