Cyclic pressure induced decellularization of porcine descending aortas.

ABSTRACT: The demand for decellularized xenogeneic tissues used in reconstructive heart surgery has increased over the last decades. Complete decellularization of longer and tubular aortic sections suitable for clinical application has not been achieved so far. The present study aims at analyzing the effect of pressure application on decellularization efficacy of porcine aortas using a device specifically designed for this purpose. Fresh porcine descending aortas of 8 cm length were decellularized using detergents. To increase decellularization efficacy, detergent treatment was combined with pressure application and different treatment schemes. Quantification of penetration depth as well as histological staining, scanning electron microscopy, and tensile strength tests were used to evaluate tissue s

SUBMITTER: Messner B 

PROVIDER: S-EPMC10115674 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature