Clinical relevance of PD-1 positive CD8 T-cells in gastric cancer.

ABSTRACT:

Background

We evaluated the relevance of PD-1⁺CD8⁺ T-cells in gastric cancer (GC) including prognostic significance, association with chemotherapy and immunotherapy sensitivity and correlations with the tumor microenvironment (TME).

Methods

Discovery cohort: GC samples were evaluated for AE1/3, CD8, PD-1, Ki-67 and Granzyme-B expression with fluorescence-based multiplex immunohistochemistry (mIHC). Validation cohorts: we analyzed bulk RNAseq GC datasets from TCGA, the "3G" chemotherapy trial and an immunotherapy phase 2 trial. The cox proportional hazards model was used to identify factors that influenced overall survival (OS). To study the TME, we analyzed single-cell RNAseq performed on GCs.

Results

In the discovery cohort of 350 GCs, increased

SUBMITTER: Choo J 

PROVIDER: S-EPMC10115710 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature