NMR-guided directed evolution.

ABSTRACT: Directed evolution is a powerful tool for improving existing properties and imparting completely new functionalities to proteins^1-4. Nonetheless, its potential in even small proteins is inherently limited by the astronomical number of possible amino acid sequences. Sampling the complete sequence space of a 100-residue protein would require testing of 20¹⁰⁰ combinations, which is beyond any existing experimental approach. In practice, selective modification of relatively few residues is sufficient for efficient improvement, functional enhancement and repurposing of existing proteins⁵. Moreover, computational methods have been developed to predict the locations and, in certain cases, identities of potentially productive mutations^6-9. Importantly, a

SUBMITTER: Bhattacharya S 

PROVIDER: S-EPMC10116341 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature