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Synthesis of Carlactone Derivatives to Develop a Novel Inhibitor of Strigolactone Biosynthesis.

ABSTRACT: Strigolactones (SLs), phytohormones that inhibit shoot branching in plants, promote the germination of root-parasitic plants, such as Striga spp. and Orobanche spp., which drastically reduces the crop yield. Therefore, reducing SL production via chemical treatment may increase the crop yield. To design specific inhibitors, it is valid to utilize the substrate structure of the target proteins as lead compounds. In this study, we focused on Os900, a rice enzyme that oxidizes the SL precursor carlactone (CL) to 4-deoxyorobanchol (4DO), and synthesized 10 CL derivatives. The effects of the synthesized CL derivatives on SL biosynthesis were evaluated by the Os900 enzyme assay in vitro and by measuring 4DO levels in rice root exudates. We identified some CL derivatives that inhibited SL biosynthesis in vitro and in vivo.

SUBMITTER: Kawada K

PROVIDER: S-EPMC10116532 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Synthesis of Carlactone Derivatives to Develop a Novel Inhibitor of Strigolactone Biosynthesis.

Kawada Kojiro K Saito Tatsuo T Onoda Satoshi S Inayama Takuma T Takahashi Ikuo I Seto Yoshiya Y Nomura Takahito T Sasaki Yasuyuki Y Asami Tadao T Yajima Shunsuke S Ito Shinsaku S

ACS omega 20230405 15

Strigolactones (SLs), phytohormones that inhibit shoot branching in plants, promote the germination of root-parasitic plants, such as <i>Striga</i> spp. and <i>Orobanche</i> spp., which drastically reduces the crop yield. Therefore, reducing SL production via chemical treatment may increase the crop yield. To design specific inhibitors, it is valid to utilize the substrate structure of the target proteins as lead compounds. In this study, we focused on Os900, a rice enzyme that oxidizes the SL p ...[more]

PMID: 37091382

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