Dataset Information

Sample Preparation of Atherosclerotic Plaque for SAXS/WAXS Experimentation.

ABSTRACT: Atherosclerosis is often described as a single disease entity; however, the morphology of each plaque is unique to the individual. The field currently lacks a technique that can discriminate stable from unstable plaques, to identify those at risk of a thromboembolic event. Small- and wide-angle X-ray scattering (SAXS/WAXS) holds the potential to be able to identify key materials present in a plaque, such as cholesterol species, collagen, low-density lipoproteins (LDLs), and hydroxyapatite. Protocols have been established for the preparation of excised human atherosclerotic tissue that are investigated herein. This includes the fixing, sectioning, and substrate selection of the sample. Through several sample preparation methods, vast improvements have been made to sample-to-noise ratio and background subtraction.

SUBMITTER: Mackley RR

PROVIDER: S-EPMC10116636 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Sample Preparation of Atherosclerotic Plaque for SAXS/WAXS Experimentation.

Mackley Rebecca R RR Huband Steven S Schiller Tara L TL

ACS omega 20230404 15

Atherosclerosis is often described as a single disease entity; however, the morphology of each plaque is unique to the individual. The field currently lacks a technique that can discriminate stable from unstable plaques, to identify those at risk of a thromboembolic event. Small- and wide-angle X-ray scattering (SAXS/WAXS) holds the potential to be able to identify key materials present in a plaque, such as cholesterol species, collagen, low-density lipoproteins (LDLs), and hydroxyapatite. Proto ...[more]

PMID: 37091388

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Project description:Electrospinning is a versatile technique to produce nanofibrous membranes with applications in filtration, biosensing, biomedical and tissue engineering. The structural and therefore physical properties of electrospun fibers can be finely tuned by changing the electrospinning parameters. The large parameter window makes it challenging to optimize the properties of fibers for a specific application. Therefore, a fundamental understanding of the multiscale structure of fibers and its correlation with their macroscopic behaviors is required for the design and production of systems with dedicated applications. In this study, we demonstrate that the properties of poly(vinylidene fluoride-co-hexafluoro propylene) (PVDF-HFP) electrospun fibers can be tuned by changing the rotating drum speed used as a collector during electrospinning. Indeed, with the help of multiscale characterization techniques such as scanning electron microscopy (SEM), small-angle X-ray scattering (SAXS), and wide-angle X-ray scattering (WAXS), we observe that increasing the rotating drum speed not only aligns the fibers but also induces polymeric chain rearrangements at the molecular scale. Such changes result in enhanced mechanical properties and an increase of the piezoelectric β-phase of the PVDF-HFP fiber membranes. We detect nanostructural deformation behaviors when the aligned fibrous membrane is uniaxially stretched along the fiber alignment direction, while an increase in the alignment of the fibers is observed for randomly aligned samples. This was analyzed by performing in situ SAXS measurements coupled with uniaxial tensile loading of the fibrous membranes along the fiber alignment direction. The present study shows that fibrous membranes can be produced with varying degrees of fiber orientation, piezoelectric β-phase content, and mechanical properties by controlling the speed of the rotating drum collector during the fiber production. Such aligned fiber membranes have potential applications for neural or musculoskeletal tissue engineering.

Dataset Information

Sample Preparation of Atherosclerotic Plaque for SAXS/WAXS Experimentation.

Publications

Sample Preparation of Atherosclerotic Plaque for SAXS/WAXS Experimentation.

Similar Datasets

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