Media component bovine serum albumin facilitates the formation of mycobacterial biofilms in response to reductive stress.

ABSTRACT:

Background

Mycobacterium tuberculosis (Mtb) forms physiologically relevant biofilms harboring drug-tolerant bacteria. This observation has brought the study of mycobacterial biofilms to the forefront of tuberculosis research. We established earlier that dithiothreitol (DTT) mediated reductive stress induces cellulose-rich biofilm formation in Mtb cultures. The molecular events associated with the DTT-induced biofilm formation are not known. Furthermore, there are only limited tools for monitoring the presence of cellulose in biofilms.

Results

To decipher the molecular events associated with DTT-induced biofilm formation, we used Mtb and non-pathogenic, fast-growing Mycobacterium smegmatis (Msm). We observed that DTT induces biofilm formation in Msm cultures. We explored whet

SUBMITTER: Mavi PS 

PROVIDER: S-EPMC10116703 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature