Identification of ferroptosis-related genes in male mice with sepsis-induced acute lung injury based on transcriptome sequencing.

ABSTRACT:

Background

Sepsis can result in acute lung injury (ALI). Studies have shown that pharmacological inhibition of ferroptosis can treat ALI. However, the regulatory mechanisms of ferroptosis in sepsis-induced ALI remain unclear.

Methods

Transcriptome sequencing was performed on lung tissue samples from 10 sepsis-induced mouse models of ALI and 10 control mice. After quality control measures, clean data were used to screen for differentially expressed genes (DEGs) between the groups. The DEGs were then overlapped with ferroptosis-related genes (FRGs) to obtain ferroptosis-related DEGs (FR-DEGs). Subsequently, least absolute shrinkage and selection operator (Lasso) and Support Vector Machine-Recursive Feature Elimination (SVM-RFE) were used to obtain key genes. In addition, Ingen

SUBMITTER: Hu W 

PROVIDER: S-EPMC10116744 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature