Dataset Information

Modeling the microbial pretreatment of camelina straw and switchgrass by Trametes versicolor and Phanerochaete chrysosporium via solid-state fermentation process: A growth kinetic sub-model in the context of biomass-based biorefineries.

ABSTRACT: Advancing microbial pretreatment of lignocellulose has the potential not only to reduce the carbon footprint and environmental impacts of the pretreatment processes from cradle-to-grave, but also increase biomass valorization, support agricultural growers, and boost the bioeconomy. Mathematical modeling of microbial pretreatment of lignocellulose provides insights into the metabolic activities of the microorganisms as responses to substrate and environment and provides baseline targets for the design, development, and optimization of solid-state-fermentation (SSF) bioreactors, including substrate concentrations, heat and mass transfer. In this study, the growth of Trametes versicolor 52J (TV52J), Trametes versicolor m4D (TVm4D), and Phanerochaete chrysosporium (PC) on camelina straw (CS) and switchgrass (SG) during an SSF process was examined. While TV52J illustrated the highest specific growth rate and maximum cell concentration, a mutant strain deficient in cellulose catabolism, TVm4D, performed best in terms of holocellulose preservation and delignification. The hybrid logistic-Monod equation along with holocellulose consumption and delignification models described well the growth kinetics. The oxygen uptake rate and carbon dioxide production rate were directly correlated to the fungal biomass concentration; however, a more sophisticated non-linear relationship might explain those correlations better than a linear model. This study provides an informative baseline for developing SSF systems to integrate fungal pretreatment into a large-scale, on-farm, wet-storage process for the utilization of agricultural residues as feedstocks for biofuel production.

SUBMITTER: Dao CN

PROVIDER: S-EPMC10117130 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Modeling the microbial pretreatment of camelina straw and switchgrass by <i>Trametes versicolor</i> and <i>Phanerochaete chrysosporium via</i> solid-state fermentation process: A growth kinetic sub-model in the context of biomass-based biorefineries.

Dao Cuong Ngoc CN Tabil Lope G LG Mupondwa Edmund E Dumonceaux Tim T

Frontiers in microbiology 20230406

Advancing microbial pretreatment of lignocellulose has the potential not only to reduce the carbon footprint and environmental impacts of the pretreatment processes from cradle-to-grave, but also increase biomass valorization, support agricultural growers, and boost the bioeconomy. Mathematical modeling of microbial pretreatment of lignocellulose provides insights into the metabolic activities of the microorganisms as responses to substrate and environment and provides baseline targets for the d ...[more]

PMID: 37089565

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Project description:The ability of white-rot fungi to degrade polysaccharides in lignified plant cell walls makes them a suitable reservoir for CAZyme prospects. However, to date, CAZymes from these species are barely studied, which limits their use in the set of choices for biomass conversion in modern biorefineries. The current work joined secretome studies of two representative white-rot fungi, Phanerochaete chrysosporium and Trametes versicolor, with expression analysis of cellobiohydrolase (CBH) genes, and use of the secretomes to evaluate enzymatic conversion of simple and complex sugarcane-derived substrates. Avicel was used to induce secretion of high levels of CBHs in the extracellular medium. A total of 56 and 58 proteins were identified in cultures of P. chrysosporium and T. versicolor, respectively, with 78-86% of these proteins corresponding to plant cell wall degrading enzymes (cellulolytic, hemicellulolytic, pectinolytic, esterase, and auxiliary activity). CBHI predominated among the plant cell wall degrading enzymes, corresponding to 47 and 34% of the detected proteins in P. chrysosporium and T. versicolor, respectively, which confirms that Avicel is an efficient CBH inducer in white-rot fungi. The induction by Avicel of genes encoding CBHs (cel) was supported by high expression levels of cel7D and cel7C in P. chrysosporium and T. versicolor, respectively. Both white-rot fungi secretomes enabled hydrolysis experiments at 10 FPU/g substrate, despite the varied proportions of CBHs and other enzymes present in each case. When low recalcitrance sugarcane pith was used as a substrate, P. chrysosporium and T. versicolor secretomes performed similarly to Cellic® CTec2. However, the white-rot fungi secretomes were less efficient than Cellic® CTec2 during hydrolysis of more recalcitrant substrates, such as acid or alkaline sulfite-pretreated sugarcane bagasse, likely because Cellic® CTec2 contains an excess of CBHs compared with the white-rot fungi secretomes. General comparison of the white-rot fungi secretomes highlighted T. versicolor enzymes for providing high glucan conversions, even at lower proportion of CBHs, probably because the other enzymes present in this secretome and CBHs lacking carbohydrate-binding modules compensate for problems associated with unproductive binding to lignin.

Project description:BackgroundA key focus in sustainable biofuel research is to develop cost-effective and energy-saving approaches to increase saccharification of lignocellulosic biomass. Numerous efforts have been made to identify critical issues in cellulose hydrolysis. Aerobic fungal species are an integral part of the carbon cycle, equip the hydrolytic enzyme consortium, and provide a gateway for understanding the systematic degradation of lignin, hemicelluloses, and cellulose. This study attempts to reveal the complex biological degradation process of lignocellulosic biomass by Phanerochaete chrysosporium in order to provide new knowledge for the development of energy-efficient biorefineries.ResultsIn this study, we evaluated the performance of a fungal biodegradation model, Phanerochaete chrysosporium, in wheat straw through comprehensive analysis. We isolated milled straw lignin and cellulase enzyme-treated lignin from fungal-spent wheat straw to determine structural integrity and cellulase absorption isotherms. The results indicated that P. chrysosporium increased the total lignin content in residual biomass and also increased the cellulase adsorption kinetics in the resulting lignin. The binding strength increased from 117.4 mL/g to 208.7 mL/g in milled wood lignin and from 65.3 mL/g to 102.4 mL/g in cellulase enzyme lignin. A detailed structural dissection showed a reduction in the syringyl lignin/guaiacyl lignin ratio and the hydroxycinnamate/lignin ratio as predominant changes in fungi-spent lignin by heteronuclear single quantum coherence spectroscopy.ConclusionP. chrysosporium shows a preference for degradation of phenolic terminals without significantly destroying other lignin components to unzip carbohydrate polymers. This is an important step in fungal growth on wheat straw. The phenolics presumably locate at the terminal region of the lignin moiety and/or link with hemicellulose to form the lignin-carbohydrate complex. Findings may inform the development of a biomass hydrolytic enzyme combination to enhance lignocellulosic biomass hydrolysis and modify the targets in plant cell walls.

Dataset Information

Modeling the microbial pretreatment of camelina straw and switchgrass by Trametes versicolor and Phanerochaete chrysosporium via solid-state fermentation process: A growth kinetic sub-model in the context of biomass-based biorefineries.

Publications

Modeling the microbial pretreatment of camelina straw and switchgrass by <i>Trametes versicolor</i> and <i>Phanerochaete chrysosporium via</i> solid-state fermentation process: A growth kinetic sub-model in the context of biomass-based biorefineries.

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