Descemet Stripping Only Technique for Corneal Endothelial Damage in Mice.

ABSTRACT:

Purpose

Descemet stripping only is an emerging surgical technique used to remove central Descemet membrane and corneal endothelial cells in patients with corneal endothelial disease. Here, we describe a murine model of this procedure to help facilitate basic science investigation and evaluation of postoperative outcomes using this surgical technique.

Methods

Slitlamp biomicroscopy, central corneal thickness assessment (by optical coherence tomography), and immunohistochemistry were used to assess the model through 7 weeks of follow-up.

Results

Complete removal of the endothelium and Descemet membrane was confirmed by slitlamp biomicroscopy and by histology. Central corneal thickness peaked at day 1 postinjury and then declined over the course of 2 weeks to a stable le

SUBMITTER: Nakagawa H 

PROVIDER: S-EPMC10117527 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature