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Trumpet is an operating system for simple and robust cell-free biocomputing.

ABSTRACT: Biological computation is becoming a viable and fast-growing alternative to traditional electronic computing. Here we present a biocomputing technology called Trumpet: Transcriptional RNA Universal Multi-Purpose GatE PlaTform. Trumpet combines the simplicity and robustness of the simplest in vitro biocomputing methods, adding signal amplification and programmability, while avoiding common shortcomings of live cell-based biocomputing solutions. We have demonstrated the use of Trumpet to build all universal Boolean logic gates. We have also built a web-based platform for designing Trumpet gates and created a primitive processor by networking several gates as a proof-of-principle for future development. The Trumpet offers a change of paradigm in biocomputing, providing an efficient and easily programmable biological logic gate operating system.

SUBMITTER: Sharon JA

PROVIDER: S-EPMC10119096 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Trumpet is an operating system for simple and robust cell-free biocomputing.

Sharon Judee A JA Dasrath Chelsea C Fujiwara Aiden A Snyder Alessandro A Blank Mace M O'Brien Sam S Aufdembrink Lauren M LM Engelhart Aaron E AE Adamala Katarzyna P KP

Nature communications 20230420 1

Biological computation is becoming a viable and fast-growing alternative to traditional electronic computing. Here we present a biocomputing technology called Trumpet: Transcriptional RNA Universal Multi-Purpose GatE PlaTform. Trumpet combines the simplicity and robustness of the simplest in vitro biocomputing methods, adding signal amplification and programmability, while avoiding common shortcomings of live cell-based biocomputing solutions. We have demonstrated the use of Trumpet to build all ...[more]

PMID: 37080970

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