High content screening miniaturization and single cell imaging of mature human feeder layer-free iPSC-derived neurons.

ABSTRACT: Human induced pluripotent stem cell (iPSC)-derived neurons are being increasingly used for high content imaging and screening. However, iPSC-derived neuronal differentiation and maturation is time-intensive, often requiring >8 weeks. Unfortunately, the differentiating and maturing iPSC-derived neuronal cultures also tend to migrate and coalesce into ganglion-like clusters making single-cell analysis challenging, especially in miniaturized formats. Using our defined extracellular matrix and low oxygen culturing conditions for the differentiation and maturation of human cortical neurons, we further modified neuronal progenitor cell seeding densities and feeder layer-free culturing conditions in miniaturized formats (i.e., 96 well) to decrease neuronal clustering, enhance single-cell identifi

SUBMITTER: Sharlow ER 

PROVIDER: S-EPMC10119332 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature