WDR76 regulates 5-fluorouracil sensitivity in colon cancer via HRAS.

ABSTRACT:

Background

WD repeat domain 76 (WDR76) has been reported in multiple tumors, while without relation to chemotherapy resistance. 5-fluorouracil (5-FU) is widely adopted in treating colon cancer. However, the resistance of WDR76 and 5-FU in colon cancer remains unclear.

Methods

Limma package in R software was employed to analyze the differentially expressed genes. Western blot or quantitative real-time PCR (qRT-PCR) were run to assessed the gene expression. The cytotoxic effect was determined according to cell viability assay, colony formation assay in vitro. Cell apoptosis was assayed using flow cytometry. GSEA analysis was performed to identify pathways related to the target gene. Xenografted mice model was employed to evaluate the tumor growth.

Results

Bioinformatic

SUBMITTER: Hu Y 

PROVIDER: S-EPMC10119360 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature