Differential requirements for P stalk components in activating yeast protein kinase Gcn2 by stalled ribosomes during stress.

ABSTRACT: The General Amino Acid Control is a conserved response to amino acid starvation involving activation of protein kinase Gcn2, which phosphorylates eukaryotic initiation factor 2 (eIF2α) with attendant inhibition of global protein synthesis and increased translation of yeast transcriptional activator GCN4. Gcn2 can be activated by either amino acid starvation or conditions that stall elongating ribosomes without reducing aminoacylation of tRNA, but it is unclear whether distinct molecular mechanisms operate in these two circumstances. We identified three regimes that activate Gcn2 in yeast cells by starvation-independent (SI) ribosome-stalling: treatment with tigecycline, eliminating the sole gene encoding tRNA^Arg_UCC, and depletion of translation termination fact

SUBMITTER: Gupta R 

PROVIDER: S-EPMC10120022 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature