ACT-Discover: identifying karyotype heterogeneity in pancreatic cancer evolution using ctDNA.

ABSTRACT:

Background

Liquid biopsies and the dynamic tracking of somatic mutations within circulating tumour DNA (ctDNA) can provide insight into the dynamics of cancer evolution and the intra-tumour heterogeneity that fuels treatment resistance. However, identifying and tracking dynamic changes in somatic copy number alterations (SCNAs), which have been associated with poor outcome and metastasis, using ctDNA is challenging. Pancreatic adenocarcinoma is a disease which has been considered to harbour early punctuated events in its evolution, leading to an early fitness peak, with minimal further subclonal evolution.

Methods

To interrogate the role of SCNAs in pancreatic adenocarcinoma cancer evolution, we applied whole-exome sequencing of 55 longitudinal cell-free DNA (cfDNA) samples

SUBMITTER: Huebner A 

PROVIDER: S-EPMC10120117 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature