The interplay between IGF-1R signaling and Hippo-YAP in breast cancer stem cells.

ABSTRACT: Both IGF-1R/PI3K/AKT/mTOR and Hippo pathways are crucial for breast cancer stem cells (BCSCs). However, their interplay remains unclear. Four triple negative breast cancer cell lines derived from CSC of two patient-derived xenografts (PDXs), AS-B145, AS-B145-1R, AS-B244, and AS-B244-1R, were used to elucidate the role of YAP in BCSCs. YAP silenced BCSCs were analyzed by cell proliferation, aldehyde dehydrogenase (ALDH) activity, mammosphere formation, and tumorigenesis. The effects of modulating IGF-1R and IGF-1 on YAP expression and localization were evaluated. The clinical correlation of YAP and IGF-1R signaling with the overall survival (OS) of 7830 breast cancer patients was analyzed by KM plotter. Knockdown of YAP abates the viability and stemness of BCSCs in vitro and tumorigenicity

SUBMITTER: Chan YT 

PROVIDER: S-EPMC10120239 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature