Early and late genome-wide gastric epithelial transcriptome response during infection with the human carcinogen Helicobacterpylori.

ABSTRACT: Infection of the stomach by Helicobacter pylori is a major risk factor for the development of gastric cancer. Colonization of the gastric epithelium leads to the activation of multiple disease-related signaling pathways. Serine protease HtrA represents an important secreted virulence factor that mediates cleavage of cellular junctions. However, its potential role in nuclear responses is unknown. Here, we performed a genome-wide RNA-seq analysis of polarized gastric epithelial cells infected by wild-type (wt) and ΔhtrA mutant bacteria. Fluorescence microscopy showed that H. pylori wt, but not ΔhtrA bacteria, preferably localized at cellular junctions. Our results pinpointed early (2 h) and late (6 h) transcriptional responses, with most differentially expressed g

SUBMITTER: Sharafutdinov I 

PROVIDER: S-EPMC10120309 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature