Identification of Critical Modules and Biomarkers of Ulcerative Colitis by Using WGCNA.

ABSTRACT:

Background

Ulcerative colitis (UC) is a chronic inflammatory disease of the colon and rectum that has no exact cause and is characterized by relapsing and remitting episodes. We aimed to find biomarkers of UC and its causes.

Methods

We got GSE73661 from the GEO database and used WGCNA to find DEGs that were expressed in the same way in both normal and UC samples. To identify the co-expression modules, we used Weighted Gene Co-Expression Network Analysis. Next, we selected genes that were both DEGs and parts of main modules. Later, three datasets were used to find the hub genes, and qRT-PCR was utilized to confirm the in-silico findings. Additionally, we analyzed the connection between the hub genes and the filtration of immune cells in UC. Using the databases, we made predic

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC10120594 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature