The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiation.

ABSTRACT: The upregulation of gene expression by enhancers depends upon the interplay between the binding of sequence-specific transcription factors (TFs) and DNA accessibility. DNA accessibility is thought to limit the ability of TFs to bind to their sites, while TFs can increase accessibility to recruit additional factors that upregulate gene expression. Given this interplay, the causative regulatory events underlying the modulation of gene expression during cellular differentiation remain unknown for the vast majority of genes. We investigated the binding-site resolution dynamics of DNA accessibility and the expression dynamics of the enhancers of an important neutrophil gene, Cebpa, during macrophage-neutrophil differentiation. Reporter genes were integrated in a site-specific manner in P

SUBMITTER: Long T 

PROVIDER: S-EPMC10120690 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature