LinRace: single cell lineage reconstruction using paired lineage barcode and gene expression data.

ABSTRACT: Understanding how single cells divide and differentiate into different cell types in developed organs is one of the major tasks of developmental and stem cell biology. Recently, lineage tracing technology using CRISPR/Cas9 genome editing has enabled simultaneous readouts of gene expressions and lineage barcodes in single cells, which allows for the reconstruction of the cell division tree, and even the detection of cell types and differentiation trajectories at the whole organism level. While most state-of-the-art methods for lineage reconstruction utilize only the lineage barcode data, methods that incorporate gene expression data are emerging, aiming to improve the accuracy of lineage reconstruction. However, effectively incorporating the gene expression data requires a reasonable model

SUBMITTER: Pan X 

PROVIDER: S-EPMC10120693 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature