Epidemiological and Molecular Characteristics of bla _NDM-1 and bla _KPC-2 Co-Occurrence Carbapenem-Resistant Klebsiella pneumoniae.

ABSTRACT:

Objective

Carbapenem-resistant Klebsiella pneumoniae (CRKP) has emerged and spread worldwide. It can usually cause a serious threat complicating treatment options in clinical settings. However, treatment options are limited. The present study investigates the prevalence and genetic characteristics of bla _NDM-1 and bla _KPC-2 co-harboring clinical isolates of Klebsiella pneumoniae.

Methods

In this study, Multiplex polymerase chain reaction (PCR) was performed to detect the carbapenem-resistant genes, and the broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of antibacterial drugs. The transferability of carbapenem-resistant phenotypes was examined using filter mating assays. Overall,

SUBMITTER: Rong F 

PROVIDER: S-EPMC10120834 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature