Effects of protease-assisted aqueous extraction on almond protein profile, digestibility, and antigenicity.

ABSTRACT: Almonds (Prunus dulcis) are one of the most consumed tree nuts worldwide and have been recognized as a healthy and nutritious food. Nevertheless, almonds are also a source of allergenic proteins that can trigger several mild to life-threatening allergic reactions. The effects of selected extraction conditions (aqueous vs. protease-assisted aqueous extraction) on the protein profile determined by proteomics analysis of excised SDS-PAGE gel bands, in vitro protein digestibility, and immunoreactivity of almond protein extracts, were evaluated. Proteolysis altered almond protein sequential and conformational characteristics thus affecting digestibility and antigenicity. Proteomics analysis revealed that enzymatic extraction resulted in the reduction of allergen proteins and epito

SUBMITTER: Furlan Goncalves Dias F 

PROVIDER: S-EPMC10121377 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature