An Integrative Biology Approach to Quantify the Biodistribution of Azidohomoalanine In Vivo.

ABSTRACT:

Background

Identification and quantitation of newly synthesized proteins (NSPs) are critical to understanding protein dynamics in development and disease. Probing the nascent proteome can be achieved using non-canonical amino acids (ncAAs) to selectively label the NSPs utilizing endogenous translation machinery, which can then be quantitated with mass spectrometry. We have previously demonstrated that labeling the in vivo murine proteome is feasible via injection of azidohomoalanine (Aha), an ncAA and methionine (Met) analog, without the need for Met depletion. Aha labeling can address biological questions wherein temporal protein dynamics are significant. However, accessing this temporal resolution requires a more complete understanding of Aha distribution kinetics in tissu

SUBMITTER: Saleh AM 

PROVIDER: S-EPMC10121978 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature