Premature termination of transcription is shaped by Rho and translated uORFS in <i>Mycobacterium tuberculosis</i>.

ABSTRACT: Little is known about the decisions behind transcription elongation versus termination in the human pathogen Mycobacterium tuberculosis (M.TB). By applying Term-seq to M.TB we found that the majority of transcription termination is premature and associated with translated regions, i.e., within previously annotated or newly identified open reading frames. Computational predictions and Term-seq analysis, upon depletion of termination factor Rho, suggests that Rho-dependent transcription termination dominates all transcription termination sites (TTS), including those associated with regulatory 5' leaders. Moreover, our results suggest that tightly coupled translation, in the form of overlapping stop and start codons, may suppress Rho-dependent termination. This study prov

SUBMITTER: D'Halluin A 

PROVIDER: S-EPMC10122055 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature