Transcriptomic analysis of feeder-free culture system for maintaining naive-state pluripotency in human pluripotent stem cells.

ABSTRACT:

Background

Human pluripotent stem cells (hPSCs) such as embryonic stem cells (ESCs) and induced pluripotent stem cells (PSCs) have the capacity of self-renewal and multilineage differentiation in vitro. Conventional hPSCs, which are in a primed state, can produce various types of differentiated cells. However, the variability in their degree of pluripotency and differentiation propensities, which is influenced by the inductive methods and culture conditions, limit their availability. Therefore, PSCs in a naïve state are a promising source of PSCs.

Methods

We recently developed a culture system for naïve hPSCs using an inhibitor of the NOTCH signaling pathway and a histone H3 methyltransferase disruptor. This culture system requires feeder cells for stably maintaining

SUBMITTER: Isono W 

PROVIDER: S-EPMC10122725 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature